Activity Pattern Discovery from Network Captures

Investigating insider threat cases is challenging because activities are conducted with legitimate access that makes distinguishing malicious activities from normal activities difficult. To assist with identifying non-normal activities, we propose using two types of pattern discovery to identify a person\u27s behavioral patterns in network data. The behavioral patterns serve to deemphasize normal behavior so that insider threat investigations can focus attention on potentially more relevant. Results from a controlled experiment demonstrate the highlighting of a suspicious event through the reduction of events belonging to discovered patterns. Abstract © 2016 IEEE

Magnetohydrodynamic Waves in a Partially Ionized Filament Thread

Oscillations and propagating waves are commonly seen in high-resolution observations of filament threads, i.e., the fine-structures of solar filaments/prominences. Since the temperature of prominences is typically of the order of 10^4 K, the prominence plasma is only partially ionized. In this paper, we study the effect of neutrals on the wave propagation in a filament thread modeled as a partially ionized homogeneous magnetic flux tube embedded in an homogeneous and fully ionized coronal plasma. Ohmic and ambipolar magnetic diffusion are considered in the basic resistive MHD equations. We numerically compute the eigenfrequencies of kink, slow, and Alfven linear MHD modes, and obtain analytical approximations in some cases. We find that the existence of propagating modes is constrained by the presence of critical values of the longitudinal wavenumber. In particular, the lower and upper frequency cut-offs of kink and Alfven waves owe their existence to magnetic diffusion parallel and perpendicular to magnetic field lines, respectively. The slow mode only has a lower frequency cut-off, which is caused by perpendicular magnetic diffusion and is significantly affected by the ionization degree. In addition, ion-neutral collisions is the most efficient damping mechanism for short wavelengths while ohmic diffusion dominates in the long-wavelength regime.Comment: Accepted for publication in Ap

Counting homomorphisms onto finite solvable groups

We present a method for computing the number of epimorphisms from a finitely-presented group G to a finite solvable group \Gamma, which generalizes a formula of G\"aschutz. Key to this approach are the degree 1 and 2 cohomology groups of G, with certain twisted coefficients. As an application, we count low-index subgroups of G. We also investigate the finite solvable quotients of the Baumslag-Solitar groups, the Baumslag parafree groups, and the Artin braid groups.Comment: 30 pages; accepted for publication in the Journal of Algebr

Interstellar solid hydrogen

We consider the possibility that solid molecular hydrogen is present in interstellar space. If so cosmic-rays and energetic photons cause ionization in the solid leading to the formation of H+6. This ion is not produced by gas-phase reactions and its radiative transitions therefore provide a signature of solid H2 in the astrophysical context. The vibrational transitions of H+6 are yet to be observed in the laboratory, but we have characterized them in a quantum-theoretical treatment of the molecule; our calculations include anharmonic corrections, which are large. Here we report on those calculations and compare our results with astronomical data. In addition to the H+6 isotopomer, we focus on the deuterated species (HD)+3 which is expected to dominate at low ionization rates as a result of isotopic condensation reactions. We can reliably predict the frequencies of the fundamental bands for five modes of vibration. For (HD)+3 all of these are found to lie close to some of the strongest of the pervasive mid-infrared astronomical emission bands, making it difficult to exclude hydrogen precipitates on observational grounds. By the same token these results suggest that (HD)+3 could be the carrier of the observed bands. We consider this possibility within the broader picture of interstellar medium photo-processes and we conclude that solid hydrogen may indeed be abundant in astrophysical environments

2000-2001 The Chamber Strings

https://spiral.lynn.edu/conservatory_otherseasonalconcerts/1100/thumbnail.jp

Targeting tauopathy with engineered tau-degrading intrabodies

BACKGROUND: The accumulation of pathological tau is the main component of neurofibrillary tangles and other tau aggregates in several neurodegenerative diseases, referred to as tauopathies. Recently, immunotherapeutic approaches targeting tau have been demonstrated to be beneficial in decreasing tauopathy in animal models. We previously found that passive immunotherapy with anti-tau antibody to human tau or expression of an anti-tau secreted single-chain variable fragment (scFv) in the central nervous system of a mouse model of tauopathy decreased but did not remove all tau-associated pathology. Although these and other studies demonstrate that conventional immunotherapeutic approaches targeting tau can influence tau pathogenesis, the majority of pathological tau remains in the cytosol of cells, not typically accessible to an extracellular antibody. Therefore, we reasoned targeting intracellular tau might be more efficacious in preventing or decreasing tauopathy. METHODS: By utilizing our anti-tau scFv, we generated anti-tau intrabodies for the expression in the cytosol of neurons. To enhance the degradation capacity of conventional intrabodies, we engineered chimeric anti-tau intrabodies fused to ubiquitin harboring distinct mutations that shuttle intracellular tau for either the proteasome or lysosomal mediated degradation. To evaluate the efficacy in delaying or eliminating tauopathy, we expressed our tau degrading intrabodies or controls in human tau transgenic mice by adeno-associated virus prior to overt tau pathology and after tau deposition. RESULTS: Our results demonstrate, the expression of chimeric anti-tau intrabodies significantly reduce tau protein levels in primary neuronal cultures expression human tau relative to a non-modified anti-tau intrabody. We found the expression of engineered tau-degrading intrabodies destined for proteasomal-mediated degradation are more effective in delaying or eliminating tauopathy than a conventional intrabody in aged human tau transgenic mice. CONCLUSION: This study, harnesses the strength of intrabodies that are amendable for targeting specific domains or modifications with the cell-intrinsic mechanisms that regulate protein degradation providing a new immunotherapeutic approach with potentially improved efficacy

Plasmid Metagenome Reveals High Levels of Antibiotic Resistance Genes and Mobile Genetic Elements in Activated Sludge

The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs

A compatible exon-exon junction database for the identification of exon skipping events using tandem mass spectrum data

<p>Abstract</p> <p>Background</p> <p>Alternative splicing is an important gene regulation mechanism. It is estimated that about 74% of multi-exon human genes have alternative splicing. High throughput tandem (MS/MS) mass spectrometry provides valuable information for rapidly identifying potentially novel alternatively-spliced protein products from experimental datasets. However, the ability to identify alternative splicing events through tandem mass spectrometry depends on the database against which the spectra are searched.</p> <p>Results</p> <p>We wrote scripts in perl, Bioperl, mysql and Ensembl API and built a theoretical exon-exon junction protein database to account for all possible combinations of exons for a gene while keeping the frame of translation (i.e., keeping only in-phase exon-exon combinations) from the Ensembl Core Database. Using our liver cancer MS/MS dataset, we identified a total of 488 non-redundant peptides that represent putative exon skipping events.</p> <p>Conclusion</p> <p>Our exon-exon junction database provides the scientific community with an efficient means to identify novel alternatively spliced (exon skipping) protein isoforms using mass spectrometry data. This database will be useful in annotating genome structures using rapidly accumulating proteomics data.</p

The chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) controls cellular quiescence by hyperpolarizing the cell membrane during diapause in the crustacean Artemia

Cellular quiescence, a reversible state in which growth, proliferation, and other cellular activities are arrested, is important for self-renewal, differentiation, development, regeneration, and stress resistance. However, the physiological mechanisms underlying cellular quiescence remain largely unknown. In the present study, we used embryos of the crustacean Artemia in the diapause stage, in which these embryos remain quiescent for prolonged periods, as a model to explore the relationship between cell-membrane potential (V-mem) and quiescence. We found that V-mem is hyperpolarized and that the intracellular chloride concentration is high in diapause embryos, whereas V-mem is depolarized and intracellular chloride concentration is reduced in postdiapause embryos and during further embryonic development. We identified and characterized the chloride ion channel protein cystic fibrosis transmembrane conductance regulator (CFTR) of Artemia (Ar-CFTR) and found that its expression is silenced in quiescent cells of Artemia diapause embryos but remains constant in all other embryonic stages. Ar-CFTR knockdown and GlyH-101-mediated chemical inhibition of Ar-CFTR produced diapause embryos having a high V-mem and intracellular chloride concentration, whereas control Artemia embryos released free-swimming nauplius larvae. Transcriptome analysis of embryos at different developmental stages revealed that proliferation, differentiation, and metabolism are suppressed in diapause embryos and restored in postdiapause embryos. Combined with RNA sequencing (RNA-Seq) of GlyH-101-treated MCF-7 breast cancer cells, these analyses revealed that CFTR inhibition down-regulates the Wnt and Aurora Kinase A (AURKA) signaling pathways and up-regulates the p53 signaling pathway. Our findings provide insight into CFTR-mediated regulation of cellular quiescence and V-mem in the Artemia model